Ochratoxin A-induced cytotoxicity in liver (HepG2) cells: Impact of serum concentration, dietary antioxidants and glutathione-modulating compounds

Abbrevations: BSO, buthionine sulfoximine; CAT, catechin; DMSO, dimethyl sulfoxide; DTNB, dithio-bis-nitrobenzoic acid; EGCG, epigallocatechin gallate; FCS, foetal calf serum; GSH, glutathione; IARC, international agency for research on cancer; NAC, N-acetylcysteine; NO, nitric oxide; NR, neutral red; OATP, organic anion-transporting polypeptide; OTA, ochratoxin A; PBS, phosphate buffered saline; QUE, quercetin; ROS, reactive oxygen species; ROSAC, rosmarinic acid; RPMI, roswell park memorial institute; α-TOC, α-tocopherol; α-TOC-P, α-tocopherol phosphat

The UV-A and visible solar irradiance spectrum: inter-comparison of absolutely calibrated, spectrally medium resolution solar irradiance spectra from balloon- and satellite-borne measurements

International audienceWithin the framework of the ENVISAT/-SCIAMACHY satellite validation, solar irradiance spectra are absolutely measured at moderate resolution in the UV/visible spectral range (in the UV from 316.7–418 nm and the visible from 400–652 nm at a full width half maximum resolution of 0.55 nm and 1.48 nm, respectively) from aboard the azimuth-controlled LPMA/DOAS balloon gondola at around 32 km balloon float altitude. After accounting for the atmospheric extinction due to Rayleigh scattering and gaseous absorption (O3, and NO2), the measured solar spectra are compared with previous observations. Our solar irradiance is +1.6% larger than the re-calibrated Kurucz et al. (1984) solar spectrum (Fontenla et al., 1999, called MODTRAN 3.5) in the visible spectral range (435–650 nm), +1.5% larger in the (370–415 nm) wavelength interval, but -4% smaller in the UV spectral range (316.7–370 nm), when the Kurucz spectrum is convolved to the spectral resolution of our instrument. The same comparison with the SOLSPEC solar spectrum (Thuillier et al., 1997, 1998a, b) confirms the somewhat larger solar irradiance (+1.7%) measured by the balloon instrument from 435–500 nm, but not from 500–650 nm, where the SOLSPEC is -1.3% lower than MODTRAN 3.5. Comparison of the SCIAMACHY solar spectrum from channels 1 to 4 (– re-calibrated by the University of Bremen –) with MODTRAN 3.5 indicates an agreement of +0.2% in the visible spectral range (435–585 nm). With this calibration, the SCIAMACHY solar spectrum is congruent with the balloon observations (-1%) in the 316.7–370 nm wavelength range, but both are up to -5%/-3% smaller than MODTRAN 3.5 and SOLSPEC, respectively. In agreement with findings of Skupin et al. (2002) our study emphasizes that the present ESA SCIAMACHY level 1 calibration is systematically +15% larger in the considered wavelength intervals when compared to all available other solar irradiance measurements

Greater Expectations?

Physically Unclonable Functions (PUFs) are key tools in the construction of lightweight authentication and key exchange protocols. So far, all existing PUF-based authentication protocols follow the same paradigm: A resource-constrained prover, holding a PUF, wants to authenticate to a resource-rich verifier, who has access to a database of pre-measured PUF challenge-response pairs (CRPs). In this paper we consider application scenarios where all previous PUF-based authentication schemes fail to work: The verifier is resource-constrained (and holds a PUF), while the prover is resource-rich (and holds a CRP-database). We construct the first and efficient PUF-based authentication protocol for this setting, which we call converse PUF-based authentication. We provide an extensive security analysis against passive adversaries, show that a minor modification also allows for authenticated key exchange and propose a concrete instantiation using controlled Arbiter PUFs

Balloon-borne stratospheric BrO measurements: comparison with Envisat/SCIAMACHY BrO limb profiles

International audienceFor the first time, results of four stratospheric BrO profiling instruments, are presented and compared with reference to the SLIMCAT 3-dimensional chemical transport model (3-D CTM). Model calculations are used to infer a BrO profile validation set, measured by 3 different balloon sensors, for the new Envisat/SCIAMACHY (ENVIronment SATellite/SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY) satellite instrument. The balloon observations include (a) balloon-borne in situ resonance fluorescence detection of BrO (Triple), (b) balloon-borne solar occultation DOAS measurements (Differential Optical Absorption Spectroscopy) of BrO in the UV, and (c) BrO profiling from the solar occultation SAOZ (Systeme d'Analyse par Observation Zenithale) balloon instrument. Since stratospheric BrO is subject to considerable diurnal variation and none of the measurements are performed close enough in time and space for a direct comparison, all balloon observations are considered with reference to outputs from the 3-D CTM. The referencing is performed by forward and backward air mass trajectory calculations to match the balloon with the satellite observations. The diurnal variation of BrO is considered by 1-D photochemical model calculation along the trajectories. The 1-D photochemical model is initialised with output data of the 3-D model with additional constraints on the vertical transport, the total amount and photochemistry of stratospheric bromine as given by the various balloon observations. Total [Bry]=(20.1±2.5) pptv obtained from DOAS BrO observations at mid-latitudes in 2003, serves as an upper limit of the comparison. Most of the balloon observations agree with the photochemical model predictions within their given error estimates. First retrieval exercises of BrO limb profiling from the SCIAMACHY satellite instrument on average agree to around 20% with the photochemically-corrected balloon observations of the remote sensing instruments (SAOZ and DOAS). An exception is the in situ Triple profile, in which the balloon and satellite data mostly does not agree within the given errors. In general, the satellite measurements show systematically higher values below 25 km than the balloon data and a change in profile shape above about 25 km

Photonuclear Reactions of Three-Nucleon Systems

We discuss the available data for the differential and the total cross section for the photodisintegration of $^3$He and $^3$H and the corresponding inverse reactions below $E_\gamma = 100$ MeV by comparing with our calculations using realistic $NN$ interactions. The theoretical results agree within the errorbars with the data for the total cross sections. Excellent agreement is achieved for the angular distribution in case of $^3$He, whereas for $^3$H a discrepancy between theory and experiment is found.Comment: 11 pages (twocolumn), 12 postscript figures included, uses psfig, RevTe

Dynamic Searchable Encryption with Access Control

We present a searchable encryption scheme for dynamic document collections in a multi-user scenario. Our scheme features fine-grained access control to search results, as well as access control to operations such as adding documents to the document collection, or changing individual documents. The scheme features verifiability of search results. Our scheme also satisfies the forward privacy notion crucial for the security of dynamic searchable encryption schemes

Far-Infrared to Millimeter Astrophysical Dust Emission. II: Comparison of the Two-Level Systems (TLS) model with Astronomical Data

In a previous paper we proposed a new model for the emission by amorphous astronomical dust grains, based on solid-state physics. The model uses a description of the Disordered Charge Distribution (DCD) combined with the presence of Two-Level Systems (TLS) defects in the amorphous solid composing the grains. The goal of this paper is to confront this new model to astronomical observations of different Galactic environments in the FIR/submm, in order to derive a set of canonical model parameters to be used as a Galactic reference to be compared to in future Galactic and extragalactic studies. We confront the TLS model with existing astronomical data. We consider the average emission spectrum at high latitudes in our Galaxy as measured with FIRAS and WMAP, as well as the emission from Galactic compact sources observed with Archeops, for which an inverse relationship between the dust temperature and the emissivity spectral index has been evidenced. We show that, unlike models previously proposed which often invoke two dust components at different temperatures, the TLS model successfully reproduces both the shape of the Galactic SED and its evolution with temperature as observed in the Archeops data. The best TLS model parameters indicate a charge coherence length of \simeq 13 nm and other model parameters in broad agreement with expectations from laboratory studies of dust analogs. We conclude that the millimeter excess emission, which is often attributed to the presence of very cold dust in the diffuse ISM, is likely caused solely by TLS emission in disordered amorphous dust grains. We discuss the implications of the new model, in terms of mass determinations from millimeter continuum observations and the expected variations of the emissivity spectral index with wavelength and dust temperature. The implications for the analysis of the Herschel and Planck satellite data are discussed.Comment: Accepted for publication in A&A (16 pages, 9 figures, 6 tables

Lightweight and Secure PUF Key Storage Using Limits of Machine Learning

13th International Workshop, Nara, Japan, September 28 – October 1, 2011. ProceedingsA lightweight and secure key storage scheme using silicon Physical Unclonable Functions (PUFs) is described. To derive stable PUF bits from chip manufacturing variations, a lightweight error correction code (ECC) encoder / decoder is used. With a register count of 69, this codec core does not use any traditional error correction techniques and is 75% smaller than a previous provably secure implementation, and yet achieves robust environmental performance in 65nm FPGA and 0.13μ ASIC implementations. The security of the syndrome bits uses a new security argument that relies on what cannot be learned from a machine learning perspective. The number of Leaked Bits is determined for each Syndrome Word, reducible using Syndrome Distribution Shaping. The design is secure from a min-entropy standpoint against a machine-learning-equipped adversary that, given a ceiling of leaked bits, has a classification error bounded by ε. Numerical examples are given using latest machine learning results

The ACOS CO_2 retrieval algorithm – Part 1: Description and validation against synthetic observations

This work describes the NASA Atmospheric CO_2 Observations from Space (ACOS) X_(CO_2) retrieval algorithm, and its performance on highly realistic, simulated observations. These tests, restricted to observations over land, are used to evaluate retrieval errors in the face of realistic clouds and aerosols, polarized non-Lambertian surfaces, imperfect meteorology, and uncorrelated instrument noise. We find that post-retrieval filters are essential to eliminate the poorest retrievals, which arise primarily due to imperfect cloud screening. The remaining retrievals have RMS errors of approximately 1 ppm. Modeled instrument noise, based on the Greenhouse Gases Observing SATellite (GOSAT) in-flight performance, accounts for less than half the total error in these retrievals. A small fraction of unfiltered clouds, particularly thin cirrus, lead to a small positive bias of ~0.3 ppm. Overall, systematic errors due to imperfect characterization of clouds and aerosols dominate the error budget, while errors due to other simplifying assumptions, in particular those related to the prior meteorological fields, appear small